Apheon Bioscience

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Mammalian Expression: C-Terminal Tag, Codon Optimization

We offer gene synthesis and custom DNA cloning services with codon optimization for expression of a C-terminal epitope-tagged or fusion protein in mammalian cell lines. Epitopes include FLAG, MYC, HA, and V5 for immunological detection and eGFP, eYFP, DsRed, and mCherry for fluorescent detection. Our system also allows you to choose a promoter (CMV or EF-1 alpha) and selection (Neomycin, Blasticidin, or Hygromycin).

Simply enter the name of your construct, the amino acid sequence of your protein (or upload a fasta file with multiple sequences), select your choice of promotor, selectable marker, C-terminal tag and organism for codon optimization.

Important: The amino acid sequence should not have any spaces and must be between 100 and 1650 amino acids (see Description below).

Catalog Number: MECO-003 Category: Service:


Recombinant DNA with Codon Optimization!

Our comprehensive cloning service allows users to simply enter the amino acid sequence of the coding region (CDS) then choose a C-terminal epitope tag of fluorescent fusion protein, promoter, and the desired mammalian antibiotic.

Notes on Ordering: The amino acid sequence must be between 100 and 1650 amino acids.  Sequences contained in a single fasta file will be constructed with the same vector elements chosen for that order.  If you have multiple sequences and want different elements, these should be entered separately.

Based on your choices, the sequence will be codon optimized and a vector will be designed.  Once the design is complete, a pdf will be sent by email prior to the start of synthesis.  If you notice any issues with your order, please contact customer service within 24 hours.  Apheon Bioscience is not responsible for errors input or uploaded by the user after production begins.

If the desired vector elements do not meet your needs, please fill out the custom vector onboarding form and we will on-board your vector, often free of charge.

Currently, clones are delivered as a bacterial stock.