Advanced Recombinant DNA Cloning

Eliminate the complexities of traditional cloning with our US-based, automated molecular DNA cloning platform. We transform your most demanding design requirements into sequence-verified constructs ready for immediate downstream applications.

Comprehensive Cloning Solutions

Our automated platform delivers precision-engineered plasmids optimized for your host system of choice. By utilizing our “CLICK & CLONE” ordering, you eliminate the time-consuming and error-prone steps of manual design and receive sequence-verified constructs ready for immediate application. To further streamline your workflow, all clones are delivered as agar stabs, removing the need for initial retransformation.

Bacterial Expression Constructs

Engineered for high-yield protein production in E. coli. Select from a versatile range of promoters and affinity tags to optimize expression levels and simplify downstream purification.

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Mammalian Expression Constructs

Optimized for high-efficiency transfection in eukaryotic systems. Our platform enables the seamless integration of diverse promoters and N- or C-terminal fusion tags including FLAG, MYC, HA, and V5 or fluorescent reporters such as GFP, YFP, dsRed, and mCherry.

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Lentiviral Constructs

Specialized for stable cell line generation, gene therapy research, and CAR-T development. Our lentiviral suite offers flexible configurations of promoters and epitopes, engineered to meet the rigorous demands of complex genomic integration.

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The Apheon Standard: Precision, Speed, and Security

Precision automated design and assembly yield 100% sequence verified constructs.

Ready-to-Use Deliverables:

Orders include an aliquot of DNA and an agar stab eliminating the need to retransform.

Whole-Plasmid Validation:

We sequence the entire vector, not just the insert, to guarantee 100% accuracy. Each delivery includes a complete electronic sequence and alignment file for your records.

Complex Cloning Expertise:

Our platform can handle difficult constructs that other companies reject, including repetitive sequences, GC-rich regions, and unstable structural elements.

Rapid Turnaround: 

We leverage automated assembly to compress your discovery timeline, transforming digital designs into physical plasmids with fast turnaround time.

BIOSECURE Act compliant:

All cloning and data handling are conducted exclusively in our U.S. facilities to ensures BIOSECURE Act compliance and protection of your sensitive IP.

Transparent Pricing:

We offer free vector onboarding, a private ordering portal and a flat rate of $0.20 per base to clone into your proprietary or preferred vector.

How to Order

CLICK & CLONE is a simple ordering process that lets you focus on your research and eliminates cloning errors.

01

Choose & Configure

Select construct type (Bacterial Expression, Mammalian Expression, or Lentiviral) and configure your specifications

02

Submit Sequence

Paste CDS from FASTA file or upload multiple sequences with custom naming

03

Launch Project

Select promoter, antibiotic resistance, and  epitope tags to complete your order