DNA cloning made simple with Design as You Order
Our comprehensive DNA cloning service allows users to simply enter the DNA sequence of the coding sequence (CDS) insert, choose a N-terminal epitope tag (FLAG, MYC, HA or V5 or fluorescent fusion protein (eGFP, eYFP, DsRed or mCherry), a promoter (CMV or EF-1 alpha), and the desired mammalian selection (Neomycin, Hygromycin, or Blasticidin) from the dropdown selectors. The insert CDS will be cloned into a vector with the functional elements shown in the image.
Notes on Ordering: The DNA of the CDS must be a multiple of 3 and cannot have spaces to ensure the proper reading frame is designed. Adding the start (ATG) or stop (TAA, TGA, TAG) codons is optional. Multiple DNA sequences contained in a single fasta file will contain the same vector elements chosen for that order. If you have multiple sequences and want different elements, these should be entered separately.
Based on your choices, a vector will be designed, and a pdf will be sent via email prior to the start of synthesis. If you notice any issues with your vector, please contact customer service within 24 hours. Apheon Bioscience is not responsible for errors input or uploaded by the user after production begins.
If the desired vector elements do not meet your needs, please fill out the custom vector onboarding form and we will on-board your vector, often free of charge.
Currently, clones are delivered as a bacterial stab along with a plasmid DNA at 100 ng/ul.