Bacterial Expression: Affinity Tagging, Codon Optimization
Gene synthesis and custom DNA cloning services for bacterial expression and affinity purification.
Affinity tags include Glutathione S-Transferase (GST), Maltose Binding Proteins (MBP) or 6 histidine (His) tags.
Simply enter the name of your construct, the DNA sequence of your coding sequence (or upload a fasta file with multiple sequences), select your choice of promotor, selectable marker, and affinity tag.
Important: The DNA cannot have any spaces, must be a multiple of 3, and be less than 5000 nucleotides (see Description below).
Description
Bacterial Expression constructs with Codon Optimization!
Our comprehensive cloning service allows users to simply enter the the amino acid sequence of the coding region (CDS) and choose the affinity tag, promoter, and antibiotic from the dropdown selectors. Codon optimization will be done based on the E. coli class II codon frequency.
Notes on Ordering: The amino acid sequence must be between 100 and 1650 amino acids. Sequences contained in a single fasta file will be constructed with the same vector elements chosen for that order. If you have multiple sequences and want different elements, these should be entered separately.
Based on your choices, the sequence will be codon optimized and a vector will be designed. Once the design is complete, a pdf will be sent by email prior to the start of synthesis. If you notice any issues with your order, please contact customer service within 24 hours. Apheon Bioscience is not responsible for errors input or uploaded by the user after production begins.
If the desired vector elements do not meet your needs, please fill out the custom vector onboarding form and we will on-board your vector, often free of charge.
Currently, clones are delivered as a bacterial stock.
