10-Beta High-Efficiency Electro-Competent

10B cells are an ideal competent cell line useful for all types of cloning.

  • 5 x 100ul Aliqouts for upto 20 transfromations
  • >1 x 10^9 Efficiency
  • Ideal for library construction and routine cloning
  • Genotype: F mcrA Δ(mrrhsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galgalnuprpsL λ
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Apheon Bioscience’s Aphe10B electrocompetent cells are optimized for high efficiency transformations. Cells are ideal for transformations of cDNA library preparations, methylated genomic DNA, difficult-to-clone genes or routine cloning where high numbers of transformants are desired. The Aphe10B genotype is similar to other commonly used 10 beta competent cells offering blue/white selection on agar plates containing X-gal. They also provide high yield and high quality plasmid DNA for subsequent experiments.

Download Product information sheet and protocol.